Signalling Intermediates and Protein Interactions Regulating Neurogenesis and Depression

Signalling Intermediates and Protein Interactions Regulating Neurogenesis and Depression

All positions have been filled

ESR5: Estabilishment of in vivo RNAi screens for MAPK8 regulators in neurogenesis

Supervisor:   Michael Courtney
Host institution:   University of Turku, Finland
Duration:  36 months

Description:
This project involves application of high-throughput methods to develop RNAi libraries suitable for stable and traceable knockdown, and apply in vivo selection to iteratively simplify the library and identify kinase regulators with most potent influence on different steps in neurogenesis. In parallel the activation of kinase pathway effectors with be addressed by development of activator trap methods. Imaging-based approaches will be used intensively in this project.
Applicants should be familiar with molecular biology and cell signalling methods. Candidates with demonstrated prior experience with high-throughput methods and/or instrumentation, electroporation, RNAi, transposases, animal models and/or neurogenesis will be at a strong advantage.

ER3: Design and validation of inhibitors of NMDA receptor signalling complexes

Supervisor:   Michael Courtney
Host institution:   University of Turku, Finland
Duration:  24 months

Description:
This project aims to characterise protein complexes mediating nNOS signalling. Using this information, protein-interaction screens will be developed, small molecules targeting protein interaction identified and thereby disrupting downstream signalling will be identified, and their impact on downstream signalling and nNOS-mediated actions on neurodegeneration and depression will be evaluated. Imaging-based approaches will be used intensively in this project.See Cao et al 2005 JCB PMID: 15631993; Florio et al 2009 B J Pharm PMID: 19732061; Doucet et al., 2012 Pharmacol Ther PMID: 22133842; Li et al. 2013 J Neurosci PMID: 23658158.

Applicants should have demonstrated prior experience in several of the following: molecular biology, cell signalling, protein expression and purification, protein interaction assays, in silico screening, high-throughput methods, neurodegeneration assays, and different cell imaging modalities.