Phage Antibody Generation for Fluorescence, MRI and PET Imaging of Neurogenesis

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Phage Antibody Generation for Fluorescence, MRI and PET Imaging of Neurogenesis

ESR9:  Development of phage antibodies for imaging plasma membrane biomarkers.

This application has been filled.

Supervisor:   Carl Borrebaeck
Host institution:   Immunovia

We seek an enthusiastic young researcher that will learn and exploit antibody engineering technologies such as those that use combinatorial library phage display to identify and subsequently perfect antigen-specific antibodies. Antibodies with properties appropriate for particular applications, for instance brain imaging or immunochemical analysis, will be developed by use of such technologies within this project. Recombinant proteins will be produced in appropriate prokaryotic or eukaryotic expression systems and modified as appropriate for the intended final application. The project involves close collaboration with the groups of Peter James at Lund University (Sweden), working on proteomics and mass spectrometry, Eleanor Coffey at Turku Centre for Biotechnology, Åbo Akademi University (Finland), working on production of recombinant protein targets, and Thomas Frodl at Trinity College Institute of Neuroscience (Ireland), working on applications of antibodies in magnetic resonance imaging.

The successful candidate should have strong expertise in molecular biology and protein chemistry and should have excellent organizational and communication skills. Experience with any of the following is strongly desired: antibody structure and function, combinatorial library design, antibody screening assays and phage display. Familiarity with laboratory automation and management software is a plus.

The aim is also to help identify cell surface markers of newly born neurons in adult brain. This project aims to generate new mechanistic knowledge on how the process of neurogenesis is regulated, as well as developing new imaging tools to monitor neurogenesis in vivo. Once markers are identified, antibodies will be raised and validated as specific markers of the neurogenic population.

The position involves a structured training program including international courses and lab placements. The successful candidate will participate in the Initial Training Network events including training workshops and symposia.